| Autor: |
Hua Han, Yayi Xia, Shuanke Wang, Bin Zhao, Zhengyi Sun, Lingwei Yuan |
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| Zdroj: |
Archives of Orthopaedic & Trauma Surgery; Jun2011, Vol. 131 Issue 6, p829-839, 11p, 1 Color Photograph, 1 Chart, 4 Graphs |
| Abstrakt: |
Introduction: Galectin-1 (Gal-1), a carbohydrate-binding protein, is differentially expressed by various normal and pathological tissues and appears to be functionally polyvalent. Recent evidence indicates that Gal-1 is involved in the proliferation of adult neural progenitor cells in neurogenic regions during adulthood. However, localization and functional roles of Gal-1 in the adult spinal cord have not been clarified. Method: Here, we investigated the spatio-temporal profile of endogenous Gal-1 expression by in situ hybridization before and after experimental adult spinal cord injury and examined the correlation of Gal-1 with the fate of dividing cells in vivo, using double-labeling methods. Gal-1 mRNA was detectable at a relatively low level in uninjured spinal cord, but was markedly increased in the gray matter and/or white matter and in the ependyma rostral and caudal to the lesion site after injury. Results: Co-localization results revealed that Gal-1 was expressed predominantly by GFAP-positive reactive astrocytes. In addition, intrathecal infusion of recombinant Gal-1 enhanced cell division and reactive astrocytosis in the adult spinal cord. To explore further whether Gal-1 and reactive astrocytes provide a synergistic effect on neurological recovery following SCI, we investigated the differences in behavioral analysis between wild-type (WT) and reactive astrocyte-deficient transgenic mice after injury and found neuroprotective effects of Gal-1 appeared to be specifically mediated through reactive astrocytes. Conclusion: These results indicate that Gal-1 exhibits great potential as a novel neuroprotective agent for the treatment of SCI. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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