| Autor: |
Ying-Bo Mao, Xiao-Yuan Tao, Xue-Yi Xue, Ling-Jian Wang, Xiao-Ya Chen |
| Zdroj: |
Transgenic Research; Jun2011, Vol. 20 Issue 3, p665-673, 9p, 1 Color Photograph, 4 Graphs |
| Abstrakt: |
RNA interference (RNAi) plays an important role in regulating gene expression in eukaryotes. Previously, we generated Arabidopsis and tobacco plants expressing double-stranded RNA (dsRNA) targeting a cotton bollworm ( Helicoverpa armigera) P450 gene, CYP6AE14. Bollworms fed on transgenic dsCYP6AE14 plants showed suppressed CYP6AE14 expression and reduced growth on gossypol-containing diet (Mao et al., in Nat Biotechnol 25: 1307-1313, ). Here we report generation and analysis of dsRNA-expressing cotton ( Gossypium hirsutum) plants. Bollworm larvae reared on T2 plants of the ds6- 3 line exhibited drastically retarded growth, and the transgenic plants were less damaged by bollworms than the control. Quantitative reverse-transcription polymerase chain reaction (RT-PCR) showed that the CYP6AE14 expression level was reduced in the larvae as early as 4 h after feeding on the transgenic plants; accordingly, the CYP6AE14 protein level dropped. These results demonstrated that transgenic cotton plants expressing dsCYP6AE14 acquired enhanced resistance to cotton bollworms, and that RNAi technology can be used for engineering insect-proof cotton cultivar. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Full text from SpringerLink