| Autor: |
Qiuling Xie, Michel, Patrik, Baldi, Lucia, Hacker, David, Xiaowei Zhang, Wurm, Florian |
| Předmět: |
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| Zdroj: |
Biotechnology Letters; May2011, Vol. 33 Issue 5, p897-902, 6p |
| Abstrakt: |
Here we present the TubeSpin bioreactor 50 (TubeSpins) as a simple and disposable culture system for Sf-9 insect cells in suspension. Sf-9 cells had substantially better growth in TubeSpins than in spinner flasks. After inoculation with 10 cells/ml, maximal cell densities of 16 × 10 and 6 × 10 cells/ml were reached in TubeSpins and spinner flasks, respectively. In addition the cell viability in these batch cultures remained above 90% for 10 days in TubeSpins but only for 4 days in spinner flasks. Inoculation at even higher cell densities reduced the duration of the lag phase. After inoculation at 2.5 × 10 cells/ml, the culture reached the maximum cell density within 3 days instead of 7 days as observed for inoculation with 10 cells/ml. Infection of Sf-9 cells in TubeSpins or spinner flasks with a recombinant baculovirus coding for green fluorescent protein (GFP) resulted in similar GFP-specific fluorescence levels. TubeSpins are thus an attractive option for the small-scale cultivation of Sf-9 cells in suspension and for baculovirus-mediated recombinant protein production. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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