| Autor: |
Romero, Lucía Virginia, Targovnik, Alexandra, Wolman, Federico Javier, Cascone, Osvaldo, Miranda, María Victoria |
| Předmět: |
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| Zdroj: |
Biotechnology Letters; May2011, Vol. 33 Issue 5, p947-956, 10p |
| Abstrakt: |
process based on orally-infected Rachiplusia nu larvae as biological factories for expression and one-step purification of horseradish peroxidase isozyme C (HRP-C) is described. The process allows obtaining high levels of pure HRP-C by membrane chromatography purification. The introduction of the partial polyhedrin homology sequence element in the target gene increased HRP-C expression level by 2.8-fold whereas it increased 1.8-fold when the larvae were reared at 27°C instead of at 24°C, summing up a 4.6-fold overall increase in the expression level. Additionally, HRP-C purification by membrane chromatography at a high flow rate greatly increase D the productivity without affecting the resolution. The V and K values of the recombinant HRP-C were similar to those of the HRP from Armoracia rusticana roots. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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