| Autor: |
Chenyang Ji, Fuli Min, Weiqiang Liang, Yuhong Chen, Shujuan Pan, Lianjie Bin, Shihong Chen, Zhe Wei, Tao He, Yanhui Gu, Jinming Zhang |
| Předmět: |
|
| Zdroj: |
BJU International; May2011, Vol. 107 Issue 10, p1638-1646, 9p, 11 Color Photographs, 3 Black and White Photographs, 1 Diagram, 1 Graph |
| Abstrakt: |
OBJECTIVE • To investigate the feasibility of tissue-engineered corpus cavernosum (TECC) with muscle-derived stem cells (MDSCs) as seed cells and determine the growth potential in vivo . MATERIALS AND METHODS • Acellular corporal collagen matrices (ACCMs) were obtained from adult rabbit penis by a cell removal procedure. MDSCs were separated and purified using a digestion method and Preplate technique, then seeded on ACCMs at a concentration of 30 × 106 cells/mL to construct TECCs. After 5 days of culture, seeded ACCMs were implanted with albuginea of rabbits. The implants were retrieved at 2, 4 and 6 months after implantation. • Histochemistry, immunohistochemisry and scanning electron microscopy were performed to analyse the morphological characteristics of the TECCs. RESULTS • The decellularization process successfully extracted all cellular components while preserving the original collagen fibres. • Histological analyses of the explants at all time points in the experimental group had more cells and better arranged growth than the control group. á -Smooth muscle actin and endothelial nitric oxide synthasepositive cells were more prevalent in the experimental group. CONCLUSION • Our study showed that MDSCs can be seeded on three-dimensional ACCM scaffolds and develop tissues that are similar to native normal corpus cavernosum. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
