Up-regulation of antioxidant enzymes and coenzyme Q10 in a human oral cancer cell line with acquired bleomycin resistance.

Autor:	Yen, Hsiu-Chuan, Li, Sin-Hua, Majima, Hideyuki J., Huang, Yu-Hsiang, Chen, Chiu-Ping, Liu, Chia-Chi, Tu, Ya-Chi, Chen, Chih-Wei
Předmět:	ORAL cancer CANCER cells BLEOMYCIN DRUG resistance in cancer cells HYDROGEN peroxide OXIDATIVE stress ENZYME regulation ANTIOXIDANTS
Zdroj:	Free Radical Research; Jun2011, Vol. 45 Issue 6, p707-716, 10p, 1 Chart, 7 Graphs
Abstrakt:	Bleomycin (BLM) is an anti-cancer drug that can induce formation of reactive oxygen species (ROS). To investigate the association between up-regulation of antioxidant enzymes and coenzyme Q10 (CoQ10) in acquired BLM resistance, one BLM-resistant clone, SBLM24 clone, was selected from a human oral cancer cell line, SCC61 clone. The BLM resistance of SBLM24 clone relative to a sub-clone of SCC61b cells was confirmed by analysis of clonogenic ability and cell cycle arrest. CoQ10 levels and levels of Mn superoxide dismutase, glutathione peroxidase 1, catalase and thioredoxin reductase 1 were augmented in SBLM24 clone although there was also a mild increase in the expression of BLM hydrolase. Suppression of CoQ10 levels by 4-aminobenzoate sensitized BLM-induced cytotoxicity. The results of suppression on enhanced ROS production by BLM and the cross-resistance to hydrogen peroxide in SBLM24 clone further demonstrated the development of adaptation to oxidative stress during the formation of acquired BLM resistance. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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