| Autor: |
Jung, Sung-Ju, Im, Eun-Young, Strüder-Kypke, Michaela, Kitamura, Shin-Ichi, Woo, Patrick |
| Předmět: |
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| Zdroj: |
Parasitology Research; May2011, Vol. 108 Issue 5, p1153-1161, 9p |
| Abstrakt: |
The scuticociliate Miamiensis avidus is a histophagous parasite that causes high mortality in cultured marine fishes. Small subunit ribosomal RNA (SSU rRNA) and mitochondrial cytochrome c oxidase subunit 1 ( cox1) genes were analyzed for 21 strains of M. avidus isolated from diseased olive flounder ( Paralichthys olivaceus), ridged-eye flounder ( Pleuronichthys cornutus), and spotted knifejaw ( Oplegnathus fasciatus) in Korea and Japan (collected in 2003-2007). Analysis of SSU rRNA gene sequences (1,759 bp) indicates they are very conserved with less than 0.17% (3 nucleotides) differences suggesting that SSU rRNA are useful to identify M. avidus; however, the cox1 gene (900 bp) has higher variations with intraspecific divergences up to 5.67% (51 nucleotides). A distance tree of cox1 gene sequences based on a neighbor-joining analysis can separate 21 strains into five cox1 types (two heterogeneous clusters and three individual branches). The cox1-type matches with serotype of strains but do not reflect geographical origins, host species, or pathogenicity. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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