| Autor: |
Tabbene, Olfa, Karkouch, Ines, Slimene, Imen Ben, Elfeddy, Najib, Cosette, Pascal, Mangoni, Maria-Luisa, Jouenne, Thierry, Limam, Ferid |
| Zdroj: |
Applied Biochemistry & Biotechnology; May2011, Vol. 164 Issue 1, p34-44, 11p, 3 Charts, 4 Graphs |
| Abstrakt: |
When cultured in minimal growth medium, the B38 strain of Bacillus subtilis did not exhibit any antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) clinical isolate. Coculturing B38 strain with viable MRSA cells weakly increased antibacterial activity production (20 AU/ml). Addition of dead MRSA cells in a B38 culture, increased by 8-fold the B. subtilis strain antibacterial activity reaching 160 AU/ml against MRSA strain. This antibacterial activity recovered from cell-free supernatants was stimulated by an autoinducing compound which is sensitive to the action of proteinase K suggesting a proteinaceous nature. This compound was heat-stable till 80 °C and showed a molecular mass around 20 kDa as determined by SDS-PAGE. These results suggest that the production of antibacterial compounds by B38 strain is dependent on the amount of the autoinducing compound. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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