| Autor: |
Kirkpatrick, Lisa J., Rosser, Benjamin W. C., Webb, Samuel M., Nichol, Helen |
| Předmět: |
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| Zdroj: |
Synchrotron Radiation News; Nov/Dec2009, Vol. 22 Issue 6, p33-37, 5p, 3 Color Photographs, 1 Diagram |
| Abstrakt: |
Hard X-ray Scanning Microprobe Analysis in X-ray fluorescence (XRF) mode is a sensitive tool to simultaneously map the distribution and quantity of multiple elements in tissues. Elements not readily detected by histological techniques can be visualized, and the interrelationships of multiple elements can be studied. Obtaining optimal results often depends more on the way the samples are prepared than on beamline characteristics. Freeze-drying (lyophilizing) is a well-established technique for preparing biological tissues as applied to XRF analysis of hard tissues such as bone and plant tissue [1, 2], but it is not the technique of choice for frozen sections of soft tissue [3]. In this article, we describe a straightforward method to prepare freeze-dried sections and demonstrate the importance of freeze-drying to obtain optimum cellular detail in a highly organized soft tissue. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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