Effect of Diet Hardness on Mandibular Condylar Cartilage Metabolism.
| Autor: | Tiilikainen, Petri, Raustia, Aune, Pirttiniemi, Pertti |
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| Předmět: |
CARTILAGE
METABOLISM DIET HARDNESS METALLOPROTEINASES IMMUNOHISTOCHEMISTRY CELLS COLLAGEN LABORATORY rats ANALYSIS of variance HYPOTHESIS ANIMAL experimentation COMPUTER software ENZYMES HISTOLOGICAL techniques LONGITUDINAL method MANDIBULAR condyle RATS STATISTICS DATA analysis STATISTICAL significance INTER-observer reliability |
| Zdroj: | Journal of Orofacial Pain; Winter2011, Vol. 25 Issue 1, p68-74, 7p, 1 Color Photograph, 1 Diagram, 1 Chart, 2 Graphs |
| Abstrakt: | Aims: To study the effect of diet hardness on condylar cartilage thickness, extracellular matrix composition, and expression of matrix metalloproteinase (MMP) -3, -8 and tissue inhibitor of metalloproteinase-1 (TIMP-1), by using immunohistochemical and morphometric methods. Methods: Seventy-two female Sprague Dawley rats were exposed to different dietary hardness, from soft to hard. MMP -3, -8, and TIMP-1 expression, cartilage thickness, cell count, and expression of type II collagen were studied. Analysis of variance among treatments was carried out followed by Bonferroni's comparisons test. Results: The ratio of MMP-3 and TIMP-1 immunopositive cartilage cells were similar in all age groups, whereas the number of MMP-8 positive cells decreased with age. A change of diet from soft to hard caused a significant decrease in the number of MMP-3 and MMP-8 and an increase in TIMP-1 positive cells. Cartilage thickness and area of type II collagen-positive staining were significantly affected by diet hardness. Conclusion: The results show that a soft diet during growth increases collagenolytic activity and may increase the vulnerability of condylar cartilage. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
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