| Autor: |
Raffin-Sanson, Marie-Laure, Massias, Jean Francis, Ankotche, Amos, Coste, Joël, de Keyzer, Yves, Oliver, Charles, Dumont, Chantal, Cabrol, Dominique, Ferré, Francoise, Bertagna, Xavier, Raffin-Sanson |
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| Zdroj: |
Clinical Endocrinology; Jan1999, Vol. 50 Issue 1, p85-94, 10p, 2 Diagrams, 1 Chart, 8 Graphs |
| Abstrakt: |
OBJECTIVEACTH-producing non-pituitary tumours are often associated with altered precursor processing, particularly in the most aggressive ones. Since placental tissue is characterized by its ability to express the proopiomelanocortin (POMC) gene and rapid cellular proliferation, we examined whether intact POMC could be released physiologically during human gestation. SUBJECTSOne hundred and fifty six normal pregnant women, 12 with multiple pregnancies, and 23 non-pregnant controls. Twenty-eight women were studied in the immediate postpartum period. MEASUREMENTSWe measured plasma POMC levels with a specific immunoradiometric assay (IRMA) using a combination of antibodies directed against ACTH and β endorphin. Results obtained with this first IRMA were confirmed in 22 subjects with a second assay using the same β endorphin antibody and a more distal antibody directed against the N-terminal fragment of POMC. Reverse transcription-PCR detected full length, pituitary-like, POMC mRNA in human placenta. RESULTSPlasma POMC was undetectable (<60 U/ml) in 23 normal subjects. In normal monofetal pregnancies, POMC became detectable in most women by the third month and then increased steadily until mid-gestation: 168 ± 108 (U/ml; mean ± SD) between 12 and 15 weeks, 190 ± 103 between 16 and 19 weeks, 324 ± 180 between 20 and 23 weeks, 276 ± 171 between 24 and 27 weeks, 292 ± 177 between 28 and 31 weeks, 290 ± 235 between 32 and 35 weeks and 308 ± 210 between 36 weeks and parturition. Plasma POMC was significantly higher in multiple pregnancies with very high levels in three triplet-bearing mothers: 671, 941, and 1731 U/ml at 31, 33 and 32 weeks, respectively. POMC levels felt quickly in post partum, becoming undetectable in five of 13 women on day 1, seven of eight on day 2 and five of six on day 3. Plasma POMC displayed no diurnal variation, was not suppressed by glucocorticoid administration and did not correlate with plasma... [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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