| Autor: |
Uno, Masatoshi, Tsuchiyama, Junjiroh, Moriwaki, Akiyoshi, Noguchi, Toshio, Mizoguchi, Kazuhiro, Ogino, Tetsuya, Yoshino, Tadashi, Okada, Shigeru, Harada, Mine |
| Předmět: |
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| Zdroj: |
British Journal of Haematology; Jun2001, Vol. 113 Issue 4, p1009-1014, 6p, 2 Diagrams, 2 Graphs |
| Abstrakt: |
Epstein-Barr virus (EBV)-associated nasal T/natural killer (NK) cell lymphoma has often been reported in Asian countries and has been recently confirmed as a novel clinicopathological entity. The prognosis of advanced stage disease is quite poor and an effective chemotherapeutic modality is strongly advocated. We have established the novel cell line NK-YS, which preserves the original characteristics of EBV-associated nasal angiocentric T/NK cell lymphoma. Using this cell line, we investigated the induction of apoptosis by apoptosis-inducing agents, and expression of P-glycoprotein (P-gp), p53 and bcl-2 proteins. NK-YS showed resistance towards apoptosis-inducing agents and expressed bcl-2 and P-gp but not p53. To overcome this drug resistance, we added cyclosporine A (CsA) and these agents to culture media as a P-gp antagonist. The combination of CsA and etoposide or CsA and doxorubicin induced apoptotic cell death. These results indicated that P-gp-mediated drug resistance is an essential mechanism of drug resistance of the NK-YS cell line. Combined therapy of conventional anti-cancer agents with CsA may have an important place in the establishment of a curative therapy for disseminated nasal angiocentric NK cell lymphoma. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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