Isolation and characterization of the cell-surface polysaccharides of Porphyromonas gingivalis ATCC 53978.

Autor: Farquharson, S.I., Germaine, G.R., Gray, G.R.
Předmět:
Zdroj: Oral Microbiology & Immunology; Jun2000, Vol. 15 Issue 3, p151, 7p, 1 Chart, 3 Graphs
Abstrakt: The cell-surface carbohydrates of Porphyromonas gingivalis strain ATCC 53978 were isolated and partially characterized. Three separate polysaccharides were found to be present: an extracellular polysaccharide, capsular polysaccharide and lipopolysaccharide. The capsular polysaccharide, which had peculiar, gel-like viscoelastic properties, was found to be comprised of mannuronic acid, glucuronic acid, galacturonic acid, galactose, and 2-acetamido-2-deoxy-d-glucose in relative molar ratios of 0.6:0.9:0.5:0.5:1.0, respectively. The extracellular polysaccharide was found to contain mannose, rhamnose, glucose, galactose, and 2-acetamido-2-deoxy-d-glucose in relative molar ratios of 13.5:1.4:1.0:2.0:1.0, respectively. The lipopolysaccharide was found to contain an O-antigen with a regular tetrasaccharide repeat unit comprised of 4-linked a-l-rhamnopyranosyl, 6-linked a-d-glucopyranosyl, 3-linked a-d-galactopyranosyl, and 4-linked 2- acetamido-2-deoxy-b-d-glucopyranosyl residues in equimolar proportions. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index