| Autor: |
Jian-Xin Zhao, Lian Yang, Zhen-Nan Gu, Hai-Qin Chen, Feng-Wei Tian, Yong-Quan Chen, Hao Zhang, Wei Chen |
| Předmět: |
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| Zdroj: |
International Journal of Molecular Sciences; Jan2011, Vol. 12 Issue 1, p1-11, 11p, 1 Diagram, 2 Charts, 3 Graphs |
| Abstrakt: |
The interdomain instability of single-chain fragment variable (scFv) might result in intermolecular aggregation and loss of function. In the present study, we stabilized H4—an anti-aflatoxin B1 (AFB1) scFv—with an interdomain disulfide bond and studied the effect of the disulfide bond on antibody affinity. With homology modeling and molecular docking, we designed a scFv containing an interdomain disulfide bond between the residues H44 and L100. The stability of scFv (H4) increased from a GdnHCl50 of 2.4 M to 4.2 M after addition of the H44-L100 disulfide bond. Size exclusion chromatography revealed that the scFv (H44-L100) mutant existed primarily as a monomer, and no aggregates were detected. An affinity assay indicated that scFv (H4) and the scFv (H44-L100) mutant had similar IC50 values and affinity to AFB1. Our results indicate that interdomain disulfide bonds could stabilize scFv without affecting affinity. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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