| Autor: |
Zhang, Xiao-ying, Qu, Xun, Wang, Cheng-qin, Zhou, Cheng-jun, Liu, Gui-xiang, Wei, Feng-cai, Sun, Shan-zhen |
| Předmět: |
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| Zdroj: |
Acta Pharmacologica Sinica; Feb2011, Vol. 32 Issue 2, p253-258, 6p |
| Abstrakt: |
AbstractAim:To investigate the effect of the growth arrest- and DNA damage-inducible Gadd45a gene on the radiosensitivity of human tongue squamous cell carcinoma cell line to ionizing radiation (IR).Methods:Short interfering ribonucleic acid (si-RNA) targeting Gadd45a or an irrelevant mRNA (nonsense si-RNA) was chemically synthesized. The constructed si-RNAs were transfected into Tca8113 cells and Gadd45a expression was determined using quantitative real-time PCR and Western-blot. After 24-h exposure to IR at a dose rate of 4 Gy/min, apoptosis of Tca8113 cells was detected using flow cytometry, and radiosensitivity was measured using MTT assays.Results:IR apparently increased the expression of Gadd45a at mRNA and protein levels in Tca8113 cells. The effect was efficiently inhibited by transfection with Gadd45a si-RNA (P<0.01). Furthermore, silencing Gadd45a gene significantly increased cell viability and decreased the percentage of apoptotic cells during irradiation, which indicated that IR-induced Gadd45a over-expression could increase the radiosensitivity of Tca8113 cells.Conclusion:These results indicated that targeting Gadd45a may have important therapeutic implications in sensitizing Tca8113 cells to IR. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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