| Autor: |
Yotsumoto, Shinichi, Tsuyama, Shinichiro, Tashiro, Masaaki, Murata, Fusayoshi |
| Zdroj: |
Journal of Electron Microscopy; Feb1990, Vol. 39 Issue 1, p26-32, 7p |
| Abstrakt: |
Mucous glycoproteins of rat Brunner's gland were examined ultracytochemically to elucidate the intracellular localization and sites of glycosylation in relation to the functional polarity of the cell organellae using a combination of hydrophilic resin embedment and postembedding staining with labeled lectins. A few cisternae were stained with HPA ( agglutinin, specific for terminal α-N-acetylgalactosamine) and DBA ( aggulutinin, specific for terminal α-N-acetylgalactosamine). cisternae as well as cisternae following secretory granules were stained with HPA and RCA-I ( agglutinin I, specific for terminal β-galactose). UEA-I ( agglutinin I, specific for terminal α-L-fucose) seemed to bind to transmost cisternae rather than trans cisternae. HPA bound to the whole cisternae, but to the cis or cisternae depending on a cell type. Apical mucous granules were stained with UEA-I, RCA-I, and HPA, but not with DBA. DBA seemed to be a good marker of the cis side of the Golgi apparatus and cell membrane. The glycosylation of mucous glycoprotein in rat Brunner's gland was partly clarified electron microscopically. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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