| Autor: |
Kleman, Marika, Övervik, Eva, Blanck, Agneta, Gustafsson, Jan-Åke |
| Zdroj: |
Carcinogenesis; 1989, Vol. 10 Issue 9, p1697-1700, 4p |
| Abstrakt: |
The initiating activity of 2-amino-1-methyl-6-phenylimidazo-[4,5-]pyridine (PhIP), 2-amino-3,8-dimethylimidazo[4,5-]-quinoxaline (MelQx) and a mutagenic meat extract obtained from cooked meat was examined, using the resistant hepatocyte model (RH-model). Male Wistar rats were given a single i.p. injection of PhIP (50 or 75 mg/kg body weight) or MelQx (50 mg/kg body weight) after a 2/3 partial bepa-tectomy (PH). The meat extract (corresponding to 1050 g meat/animal) was given by gastric feeding at three time points after PH. Two weeks after initiation the rats received a diet containing 0.02% 2-acetylaminofluorene for a period of 2 weeks. In the middle of this period a single dose of carbon tetrachloride was given. Rats were killed 6 weeks after the experimental start. The number of enzyme-altered (σ-glutamyl-transferase positive) hepatic foci was significantly increased in the animals given MelQx ( < 0.05) and the highest dose of PhIP ( < 0.01) whereas no effect of the meat extract was observed. The mutagenic meat extract was also studied with regard to promotive capacity , the meat extract was given in the diet of diethylnitrosamine initiated rats for a period of 6 weeks. No significant changes were detected after administration of the meat extract in the diet. It is concluded that both MelQx and PhIP are weak initiators in the RH-model, in the same order of magnitude as the previously investigated, structurally related pyrolysis products. The meat extract was not active in the RH-model under the conditions used in this study. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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