Human insulin gene expressing with Bombyx mori multiple nucleopolyhedrovirus (BmMNPV) expression system.

Autor:	Wan-fu Yue, Fang Zhou, Jia-biao Hu, Enoch Park, Hull, Joe, Yun-gen Miao
Předmět:	NUCLEOPOLYHEDROVIRUSES SILKWORMS CYSTEINE proteinases GENE expression CHITINASE BACULOVIRUSES
Zdroj:	World Journal of Microbiology & Biotechnology; Feb2011, Vol. 27 Issue 2, p393-399, 7p
Abstrakt:	Using human genomic DNA as a template, the human insulin gene was cloned and used to construct various reBmMNPVbacmids. Cysteine protease gene deletion (CPD-BmMNPV bacmid) and cysteine protease- and chitinase-deficient (CPPD- BmMNPV bacmid) baculoviruses were used to express both native and FLAG-tagged human insulin. Silkworm larvae were infected with the above recombinant bacmid DNAs, and the expressed insulin was purified and identified from infected silkworm haemolymph. The highest expression was shown with the CPPD- BmMNPV bacmid, which was about two times that of the wild type of reBmMNPVbacmid, reaching 15.827 ng/ml haemolymph. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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