| Abstrakt: |
In order to study the characteristics of human acrosome reacted spermatozoa, we developed a method to select them. Spermatozoa, obtained from 20 fertile volunteers, were selected by using GB24 antibody fixed on magnetic immunobeads, after acrosome reaction induced by 50% follicular fluid. Bead-bound spermatozoa were then detached using sheep anti-mouse IGG F(ab′)2 antibody. This method allowed recovery of 170 ± 48×10 spermatozoa ( = 20), free of GB24 antibody, as assessed by incubation with FITC-rabbit anti-mouse antibody. The percentage of acrosome reacted spermatozoa in the selected population was 88 ± 3% versus 32 ± 6% in the whole sperm population. Concerning sperm morphology, the percentage of head abnormalities was lowered (15 ± 3% versus 20 ± 3%). The motility of selected spermatozoa was dramatically reduced (7 ± 3% versus 53 ± 7% in the whole population) despite no difference in viability (84 ± 3% versus 80 ± 4%). However, the viability after an 18 h incubation was very low (1 ± 0.5% versus 46 ± 5%). These results show that acrosome reaction occurs in the most morphologically normal spermatozoa and is followed by a loss in motility and a decrease in longevity. [ABSTRACT FROM PUBLISHER] |
