| Autor: |
Torfs, Herbert, Shariatmadari, Ramin, Guerrero, Felix, Parmentier, Marc, Poels, Jeroen, Poyer, Wendy Van, Swinnen, Elfriede, De Loof, Arnold, Akerman, Karl, Broeck, Jozef Vanden |
| Předmět: |
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| Zdroj: |
Journal of Neurochemistry; May2000, Vol. 74 Issue 5, p2182, 8p |
| Abstrakt: |
STKR is an insect G protein-coupled receptor, cloned from the stable fly Stomoxys calcitrans. It displays sequence similarity to vertebrate tachykinin [or neurokinin (NK)] receptors. Functional expression of the cloned STKR cDNA was obtained in cultured Drosophila mela-nogaster Schneider 2 (S2) cells. Insect tachykinin-like peptides or 'insectatachykinins,' such as Locusta tachykinin (Lom-TK) III, produced dose-dependent calcium responses in stably transfected S2-STKR cells. Vertebrate tachykinins (or neurokinins) did not evoke any effect at concentrations up to 10[supminus5] M, but an antagonist of mammalian neurokinin receptors, spantide II, inhibited the Lom-TK III-induced calcium response. Further analysis showed that the agonist-induced intracellular release of calcium ions was not affected by pretreatment of the cells with pertussis toxin. The calcium rise was blocked by the phospholipase C inhibitor U73122. In addition, Lom-TK III was shown to have a stimulatory effect on the accumulation of both inositol 1,4,5-trisphosphate and cyclic AMP. These are the same second messengers that are induced in mammalian neurokinin-dependent signaling processes. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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