| Autor: |
Guang-Jun Jing, Dong-Hui Xu, Song-Lin Shi, Qi-Fu Li, San-Ying Wang, Fu-Yun Wu, Hai-Yan Kong |
| Předmět: |
|
| Zdroj: |
Journal of Gastroenterology & Hepatology; Jan2011, Vol. 26 Issue 1, p108-115, 8p, 2 Black and White Photographs, 1 Chart, 3 Graphs |
| Abstrakt: |
Nuclear-matrix proteins can be proteomic markers for cancer lesions. The present study aimed to determine the roles of heterogeneous nuclear ribonucleoproteins-A2 and B1 (hnRNP-A2/B1) in human gastric carcinogenesis. Human gastric cancer and non-cancerous tissues were collected for immunohistochemical analysis. Proteomics technique, Western blot, laser confocal microscope, and real-time quantitative reverse transcription-polymerase chain reaction were performed to determine the aberrant expression of nuclear-matrix proteins. hnRNP-A2/B1 existed in the nuclear matrix of gastric cancer cells, and its expression was enhanced in human gastric cancer and decreased by hexamethylene bisacetamide. The colocalization of hnRNP-A2/B1 with c-myc, c-fos, p53, and Rb was translocated from the nucleolus to the cytoplasm during the differentiation of tumor cells. hnRNP-A2/B1 affected tumor cell differentiation through interaction with oncogenes and tumor-suppressor genes, and it was overexpressed in human gastric cancer. We postulate that hnRNP-A2/B1 could serve as a biomarker for the diagnosis of human gastric cancer. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Plný text