| Autor: |
Shinmoto, Hiroshi, Takeda, Mio, Matsuo, Yuji, Naganawa, Yasunori, Tomita, Shinichi, Takano-Ishikawa, Yuko |
| Předmět: |
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| Zdroj: |
Human Antibodies; 2010, Vol. 19 Issue 4, p101-105, 5p, 1 Diagram, 4 Graphs |
| Abstrakt: |
A human-mouse hybridoma clone #86 secreting IgM-class human monoclonal antibody to peanut allergen protein Ara h1 was newly established. To detect an antibody-binding sequence (epitope) on Ara h1, the monoclonal antibody #86 was reacted with multi-pin apparatus with a series of peptides synthesized from the amino acid sequence of Ara h1. The antibody #86 was found to bind to a peptide with amino acid sequence of 481EEEEDEDEEEEGSNREVRRY500. Further analysis with shorter pin-peptides with ten amino acid-long showed that the peptides reacted with the antibody #86 contained a sequence of 485DEDEEEE491. This might be an essential linear sequence of this epitope. When the 485DED487 part of the peptide was replaced by alanine, decreased binding of antibody #86 was observed. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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