| Autor: |
Legrand, Fanny, Driss, Virginie, Woerly, Gaëtane, Loiseau, Sylvie, Hermann, Emmanuel, Fournié, Jean-Jacques, Héliot, Laurent, Mattot, Virginie, Soncin, Fabrice, Gougeon, Marie-Lise, Dombrowicz, David, Capron, Monique |
| Předmět: |
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| Zdroj: |
PLoS ONE; 2009, Vol. 4 Issue 6, p1-16, 16p, 1 Color Photograph, 1 Diagram, 1 Chart, 6 Graphs |
| Abstrakt: |
Background: Eosinophils are effector cells during parasitic infections and allergic responses. However, their contribution to innate immunity has been only recently unravelled. Methodology/Principal Findings: Here we show that human eosinophils express CD3 and γδ T Cell Receptor (TCR) but not αβ TCR. Surface expression of γδTCR/CD3 is heterogeneous between eosinophil donors and inducible by mycobacterial ligands. Surface immunoprecipitation revealed expression of the full γδTCR/CD3 complex. Real-time PCR amplification for CD3, γ and δ TCR constant regions transcripts showed a significantly lower expression in eosinophils than in γδT cells. Limited TCR rearrangements occur in eosinophils as shown by spectratyping analysis of CDR3 length profiles and in situ hybridization. Release by eosinophils of Reactive Oxygen Species, granule proteins, Eosinophil Peroxidase and Eosinophil-Derived Neurotoxin and cytokines (IFN-γ and TNF-α) was observed following activation by γδTCR-specific agonists or by mycobacteria. These effects were inhibited by anti-γδTCR blocking antibodies and antagonists. Moreover, γδTCR/CD3 was involved in eosinophil cytotoxicity against tumor cells. Conclusions/Significance: Our results provide evidence that human eosinophils express a functional γδTCR/CD3 with similar, but not identical, characteristics to γδTCR from γδT cells. We propose that this receptor contributes to eosinophil innate responses against mycobacteria and tumors and may represent an additional link between lymphoid and myeloid lineages. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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