| Autor: |
ARII, Tatsuo, HAMA, Kiyoshi |
| Zdroj: |
Journal of Electron Microscopy; Aug1987, Vol. 36 Issue 4, p177-195, 19p |
| Abstrakt: |
An improved method of extracting three-dimensional information from HVTEM stereo images of thick biological specimens using a Luzex 5000 image analyzer is described. The values of coordinates of corresponding points on stereo images are determined by measuring the parallax between the stereo pair images. The errors which cannot be avoided in the case of application of parallel projection approximation to the electron micrographs are discussed by using the properties of our high voltage electron microscope (H-1250M; at 1,000 kV). Errors of obtained coordinates increase with separation from the current center of the objective lens according to image rotations and magnification changes depending on the change of value in the specimens. The errors due to the finite pixel size on the digitized Images are also discussed. An automatic determination method of the corresponding image points by the computer is proposed. The method is based on using the third tilt image as a reference and on referring other stereo images of the same specimen field, if necessary. Using the coordinates of each corresponding point thus obtained, line length, surface area and volume of the given structure are determined with some morphological approximations. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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