| Autor: |
Ling, T. K. W., Lyon, D. J., Cheng, A. F. B., French, G. L. |
| Zdroj: |
Journal of Antimicrobial Chemotherapy (JAC); Jul1994, Vol. 34 Issue 1, p65-71, 7p |
| Abstrakt: |
Three hundred and forty isolaes of ampicillin-resistant isolated from blood, bite and urine were collected during 1984 and 1988 in the Prince of Wales Hospital, Hong Kong and MICs of 21 β-lactam antibiotics were determined. β-Lactamases were identified by isoelectric focusing, and TEM-1 was found in 89.1% of isolates, PSE-1 in 3.2%, and OXA-1 in 2.9% of strains. Ten isolates (2.9%) possessed both TEM-1 and PSE-1. Five isolates (1.5%) produced only chromosomal enzymes and one produced an unidentified enzyme which focussed at pI 6.0. All but seven of 274 TEM-1 producers and all nine OXA-1 producers reacted with oligo-nucleotide probes in a colony blot technique. All isolates contained plasmids and 229 of 318 (72.0%) isolates transferred the ampicillin resistance factor. Of 42 transconjugants of TEM-1 producers selected for study, 39(92.9%) had only a single transferable plasmid bearing the TEM-1 gene. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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