| Autor: |
Wang, Huai-Xiu, Flaherty, Sean P., Swann, Nicholas J., Matthews, Colin D. |
| Zdroj: |
Human Reproduction; Jul1994, Vol. 9 Issue 7, p1265-1270, 6p |
| Abstrakt: |
The aim of this study was to evaluate the efficacy of enriching X-bearing human spermatozoa using 12-step (25–80%) discontinuous Percoll gradients. X- and Y-bearing spermatozoa were simultaneously identified in neat semen (controls) and in 80% Percoll fractions from the same samples using double-label fluorescence in-situ hybridization and chromosome-specific DNA probes. Hybridization and labelling efficiencies of 95–99% were obtained in all samples. The mean ratio of X- to Y-bearing spermatozoa in the controls was 49.0: 48.2, whereas there was a significant enrichment ( < 0.0001) of X-bearing spermatozoa in the Percoll fractions (mean X:Y ratio was 55.1:41.1 or 1.35). The ratio varied from 1.1–1.5 in individual Percoll samples. There were no significant differences in the proportions of aneuploid spermatozoa (XX, YY, XY) between the control and Percoll fractions. We conclude that 12-step discontinuous Percoll gradients enrich X-bearing spermatozoa, but the degree of enrichment is insufficient for use in preconceptional sex selection. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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