| Autor: |
Hozumi, Katsuto, Kondo, Motonari, Nozaki, Hldeki, Kobori, Akiko, Nishimura, Takashi, Nishikawa, Shin-Ichi, Sugamura, Kazuo, Habu, Sonoko |
| Zdroj: |
International Immunology; Sep1994, Vol. 6 Issue 9, p1451-1454, 4p |
| Abstrakt: |
The effects of IL-7 on the growth and differentiation of thymocytes were analyzed using murine fetal thymua organ cultures (FTOC) in the presence of mAbs specific for the conventional IL-7 receptor (1L-7R) and for the common γ(γc) chain. In FTOC, the development of CD4CD8 double-negative thymocytes to CD4+CD8+ double-positive (DP) and CD4+ or CD8+ single-positive (SP) cells was not completely blocked by adding these mAbs, although cell growth was reduced by the treatment. To define a developing stage sensitive to the mAbs, most immature thymocytes, Pgp-1 cells, were cultured in the 2-deoxyguartosine treated fetal thymus. In the presence of both mAbs in the culture, neither DP nor SP thymocytes developed whereas either of the mAbs partially blocked their development. These results indicate that the γC chain is involved in early T cell development as an indispensable subunlt of the functional IL-7 receptor complex. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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