| Autor: |
Dutra, Walderez O., Martins-Filho, Olindo A., Can¸ado, Jose R., Pinto-Dias, Joao Carlos, Brener, Zigman, Freeman, George L., Colley, Daniel G., Gazzinelll, Giovanni, Parra, Jupara C. |
| Zdroj: |
International Immunology; Apr1994, Vol. 6 Issue 4, p499-506, 8p |
| Abstrakt: |
Whole blood preparations from patients with either the indeterminate (asymptomatic) or cardiac clinical forms of chronic infection were analyzed by flow cytometry using double-labeling to identify subsets of circulating lymphocytes. Several significant differences were demonstrated between the blood lymphocyte profiles of chagaslc patients and non-chagaslc controls. Clear increase in the percentages and actual numbers of double-positive cells of the phenotype CD3/HLA-DR, as well as decrease in the percentage of CD45RA/CD4 and CD45RA/CD8 T cells, Indicate greater numbers of activated T cells circulating in the blood of infected patients. Consistent parallel increases were seen also in the B lymphocyte subset which stained double-positive for CD19/CD5. There were no significant differences in the circulation of these chronic chagaslc patients in the CD4:CD8 ratios. Also, no substantive phenotyplc differences were observed in the lymphocyte populations between the two ends of the clinical spectrum (Indeterminate versus cardiac) in chronic human Chagas' disease. These observations demonstrate that increased levels of activated T cells and CD5 B cells are present in the circulation of people with chronic Chagas' disease. These are cell phenotypes that have been associated in other conditions with autoimmune, polyclonal, and hyperlmmune responses. The specificities of these activated cells and the roles they may play in resistance or pathogenesis during chronic Chagas' disease need now to be determined. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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