| Autor: |
Bailey, N. C., Kasturi, K. N., Blackwell, T. K., Alt, F. W., Bona, C. A., Tucker, P. W. |
| Zdroj: |
International Immunology; Aug1991, Vol. 3 Issue 8, p751-760, 10p |
| Abstrakt: |
The immunoglobulin light chain Vκ1 gene family is polymorphic in murine inbred strains and this family has been subdivided into five sub-groups (Vκ1A–E). The Vκ1A sub-group contributes to ∼ 2% of the total serum immunoglobulin light chains in several mouse strains. However, It has been reported that this sub-group Is absentin New Zealand Black (NZB) mouse serum. Amino acid sequencing of myeloma proteins from this inbred mouse has shown that they belong to the Vκ1B sub-group. We report here the structure of nine functional germllne genes from NZB mice thathave high homologies to the Vκ1 A, Vκ1B, Vκ1C, and Vκ1D sub-groups. In addition, a novel germlinegene representing the prototype of a new sub-group (designated Vκ1F) has been Identified. We have Isolated different Vκ1 germline genes from a single restriction fragment length polymorphism (RFLP) fragment, as well as Identical Vgenes from two different RFLP migrating bands. Therefore, the complexity of the genes encoding the immunoglobulin variable region cannot be determined solely by RFLP analysis. Nucleotide sequence analysis of 16 Vκ1 genes which code for NZB autoantlbodies indicate that they belong to five different Vκ1 sub-groups with five hybridomas (31%)expressing the Vκ1A sub-group. Comparison of the sequences of Vκ1 genes expressed in hybridomas with corresponding germllne genes shows no somatic mutations [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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