| Autor: |
Mahon, Anne C., Gebre, Negussie, Nurlign, Ayenew |
| Zdroj: |
International Immunology; Sep1990, Vol. 2 Issue 9, p803-812, 10p |
| Abstrakt: |
We have investigated the B cell response to in leprosy patients and healthy controls. A comparison of Westemblotted proteins separated by two-dimensional gel electrophoresis and probed wlth pooled sera from LL and BT patients revealed distinct antigen recognition patterns tor the two classifications of the disease. To characterize the circulating B cells capable of producing antl-M.leprae antibodies , peripheral blood lymphocyte cultures were activated polycionally with an anti-CD3 mAb. The resulting culture supernatants were used to probe Western-blotted M.leprae proteins and contained antibody reactive with a 10 kd M.leprae antigen. This antbody was absent in stimulated culture supernatants from healthy occupational contacts or unexposed controls, suggesting the specificky of the response. Distinct repertoires of serum and culture supernatant anti-M.Ieprae antibodlea were observed when Western-blotted antigens were probed after two-dimensional gel electrophoresis. This method for assay of specific antibody production against individual components present in a complex mixture of antigens after polycional activation may be used to study the regulation of B cell activation in leprosy and other diseases. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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