| Autor: |
Furutani, Makoto, Yanagi, Yusuke, Fujisawa, Isao, Nakayama, Toshinori, Kishimoto, Hidehiro, Kulda, Kelsuke, Asano, Yoshihiro, Tada, Tomio |
| Zdroj: |
International Immunology; May1989, Vol. 1 Issue 3, p281-288, 8p |
| Abstrakt: |
We cloned and sequenced T cell receptor (TCR) α and β chain cDNA from a λgt10 library obtained from a murine I-A autoreactive helper T cell clone MS202. Two types of cDNA clones for the α chain and one for the β chain were obtained. The two α chain transcripts used two different V genes: V joined to J and V joined to J. The four V cDNA clones obtained did not have a complete sequence, lacking the leader portion. The V genomic gene segment of MS202 was revealed to contain two exons corresponding to the V cDNA sequence, and the potential RNA splicing signals between the two exons were Intact. Both of the a chain cDNA clones showed In-frame rearrangements. Immunoprecipitation of I-surfacelabeled lysate of MS202 with anti-TCR antiserum and subsequent electrophonetic analyses indicated that only one of the α chain polypeptides was expressed on the cell surface. Thus, allelic exclusion of the α chain in MS202 is achieved by post-transcriptional regulation rather than rearrangements. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
