| Autor: |
Tsuda, Hirohisa, Hasegawa, Makiko |
| Zdroj: |
Carcinogenesis; 1990, Vol. 11 Issue 4, p595-597, 3p |
| Abstrakt: |
The addition of sodium nitrite (NaNO; 5–20 mM) for 72 h to mouse BALB/c3T3 cells resulted in the induction of transformed foci (type III foci) in a dose-dependent manner. The cells isolated from the NaNO-induced transformed foci produced progressively growing tumors when inoculated into nude mice subcutaneously at an inoculum size of 1 × 10 cells per site. In contrast, the original untreated cells did not take even at an inoculum size of 1 × 10 cells per site. The possibility that NaNO might react with cellular or medium components to make carcinogenic -nitrosamines and that these might induce cell transformation was examined and almost excluded. Thus, nitrite itself seems to have a cell transforming activity. Recent evidence suggests that NO is produced by the activated macrophage of mammals. We also detected NO production in culture media in the mouse macrophage-like cell Line J774–A1 after lipopolysaccharide (LPS) treatment, and also in the human promyeloleukemia cell line HL60 after differentiation into macrophage-like cells by 12--tetradecanoyl phorbol-13-acetate and further activation by LPS. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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