| Autor: |
Vuillaume, Monique, Best-Belpomme, Martin, Lafont, René, Hubert, Michelle, Decroix, Yves, Sarasin, Alain |
| Zdroj: |
Carcinogenesis; 1989, Vol. 10 Issue 8, p1375-1381, 7p |
| Abstrakt: |
It has been previously shown that xeroderma pigmentosum (XP) skin biopsies and their established cell lines exhibit a decrease in catalase activity and enhanced formation of photo-produced HO. Several and thermodynamic results suggest that they energy of HO disproportionation produced by catalase could be sufficient to synthesize ATP with or without the help of intact mitochondria. In this paper, we first studied the properties of HO-stimulated ATP production in extracts of normal and pathological XP skin biopsies and cell lines. In acellular extracts of normal skin biopsies and/or cell lines, ATP production can be increased 2– to 3–fold, but only with a narrow range of HO concentration. In contrast, in extracts of pathological skins or cells, ATP production was only observed when using 10– to 1000–fold less HO concentration as defined for normal extracts. Similar results were noted with two cell lines derived from patients afflicted with ataxia telangiectasia (AT), and with simian virus 40 (SV40) transformed lines of normal, XP and AT cells, Although we have no proof that such a process may exist , we would like to suggest that both HO-stimulated ATP production and catalase activity are good indicators of the degree of normality or abnormality of skin biopsies and/or cell lines. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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