| Autor: |
Mandel, H.George, Straw, James A., Farmer, Peter, Martin, Janet |
| Zdroj: |
Carcinogenesis; 1989, Vol. 10 Issue 4, p757-762, 6p |
| Abstrakt: |
Relatively simple and rapid analytical procedures involving two sequential HPLC separations were developed for the isolation of methylated purines in the urine of rats administered radiolabeled methylating carcinogens. Following a dose of [N-methyle-N-nitrosourea (MNU), 7-methyladenine (mAde) was detected by chromatography as a urinary methylpurine in addition to the expected 7-methylguanine (mGua) and 3-methylademne (m (m Ade). When methyl-labeled methionine was given to rats concurrently with MNU, urinary m Gua was labeled, but no radioactivity was recovered in either of the two methyladenine fractions. The profile of urinary methylated purines following a dose of dimethyl sulfate to the rat was similar. Small amounts of 1-methyladenine (m Ade) and 3-methylguanine (m Gua were also detected in the urine. The excretion of m Ade derived from the methyl group of the carcinogen rather than from the normal precursor for methylation, implies that this adduct, like m Ade may serve as an indicator in urine for exposure to methylating carcinogens. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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