| Autor: |
Lutgerink, Jan T., Retè, Jan, Westra, J. Gerard, Welling, Maarten C., Loman, Henk, Kriek, Erik |
| Zdroj: |
Carcinogenesis; 1986, Vol. 7 Issue 8, p1359-1364, 6p |
| Abstrakt: |
The major aminofluorene-DNA derivative, found in the liver of rats after administration of the hepatocarcinogen -acetyl-2-aminofluorene and identified as -(deoxyguanosin-8-yI)-2-aminofluorene (dGuo-C8-AF), was introduced in different amounts in single-stranded øX174 DNA by reacting the DNA with tritium labeled -hydroxy-2-aminofluorene. The modified DNA was subsequently incubated in 0.1 NaOH at 37°C for increasing periods of time to convert the dGuo-C8-AF residues into their guanine imidazole ring-opened forms. The degree of conversion was determined by measuring the amount of residual -(guanin-8-yI)-2-aminofluorene in trifluoroacetic acid hydrolyzates of the alkali-treated DNA by h.p.l.c. In addition, the effect of ring opening on the biological activity of the DNA was monitored by transfecting the DNA to wild-type spheroplasts. The results indicate that the major aminofluorene-DNA adduct formed initially, which contributes little to inactivation, becomes lethal when its guanine imidazole ring is opened. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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