| Autor: |
Andrews, P.J., Quilliam, M.A., McCarry, B.E., Bryant, D.W., McCalla, D.R. |
| Zdroj: |
Carcinogenesis; 1986, Vol. 7 Issue 1, p105-110, 6p |
| Abstrakt: |
The incubation of [H]1,8-dinitropyrene with TA98NR followed by isolation of the DNA from these cells, hydrolysis of the DNA to nucleosides, butanol extraction of the hydrolysate and purification by reversed-phase liquid chromatography afforded a single product. Calf thymus DNA, after treatment with N-hydroxyl-1-amino-8-nitropyrene, was hydrolyzed, extracted and purified in a similar fashion to give a single compound which was shown to be the deoxyguanosine derivative 1-N-(2'-deoxyguanosin-8-yl)-amino-8-nitropyrene by a combination of proton n.m.r. and u.v.-vis. spectroscopy and fast atom bombardment mass spectrometry. The DNA adducts formed and exhibited identical chromatographic and chemical behavior. Under acidic or basic conditions the and adducts were converted to identical products. Reduction of the adduct gave a new, highly fluorescent product that had a fluorescent emission spectrum identical to that of 1,8-diaminopyrene. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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