| Autor: |
Schwarze, Per E., Pettersen, Erik O., Shoaib, Mohammed C., Seglen, Per O. |
| Zdroj: |
Carcinogenesis; 1984, Vol. 5 Issue 10, p1267-1275, 9p |
| Abstrakt: |
The sequential treatment of young Wistar rats with two different carcinogens (diethylnitrosamine - plus partial hepatectomy - as an initiator, and 2-acetylaminofluorene as a cytotometric selection pressure) induces the appearance of foci and nodules of liver cells which are phenotypically altered. By means of an algorithm which takes into account binuclearity as well as cell-to-cell aggregation it is possible to compute cellular ploidy distributions from flow-cytometric analysis of either hepatocyte suspensions or suspensions of hepatocytic nuclei. Cell suspensions isolated from carcinogen-treated rats can be shown to contain, already after 8 weeks, ∼70% small, diploid hepatocytes, whereas suspensions from normal or partially hepatectomized control livers contain only ∼10% diploid cells (the remainder-being mostly tetraploid). Isolated nodules, i.e., expanding clones of proliferating cells, believed to be neoplastic precursor lesions, contained almost only diploid cells. These observations suggest that the selective outgrowth of a population of small, diploid hepatocytes may be a significant early step in the development of liver cancer. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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