| Autor: |
Laaksonen, A.Mariitta, Hänninen, Osmo O.P., Mäntyjärvi, Rauno A. |
| Zdroj: |
Carcinogenesis; 1984, Vol. 5 Issue 4, p489-494, 6p |
| Abstrakt: |
Secondary cultures of newborn NMRI (nude) mouse skin fibroblasts were used as targets for transformation by the combined administration of SV40 and 3-methylcholanthrene (MC). The SV40-induced transformation was enhanced both by pre- and post-treatment with MC at concentrations which themselves were nontransforming. The short pretreatment caused a 2-fold enhancement in the transformation frequency at a low concentration of MC that allowed >50% of the cells to survive. A short post-treatment also caused a concentration dependent enhancement in SV40 transformation of the same magnitude. The long (72 h) post-treatment with MC increased virus transformation as much as 4.3-fold. In contrast, anthracene, a non-carcinogenic compound, had no effect on the viral transformation frequency. An analysis of the morphology of the foci revealed that long post-treatment with MC induced a high number of type I foci. Combination dishes lacked these foci almost completely, and the enhancement was due to the increased number of type II and type III foci. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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