| Autor: |
Morimoto, Kazushige, Tanaka, Akria, Yamaha, Tsutomu |
| Zdroj: |
Carcinogenesis; 1983, Vol. 4 Issue 11, p1455-1458, 4p |
| Abstrakt: |
[-propyl-2,3-H]1--Propyl-1-nitrosourea (PNU) (sp. act.: 665 mCi/mmol) was synthesized and incubated with calf thy-mus DNA at pH 7.0 and 37°C . The degree of alkylation was proportional to the concentration of PNU added. The extent was 68 pmol/mg of DNA at 1 mM PNU. After mild add hydrolysis, purine bases were separated by Sephadex G-10 column chromatography. Although O--propylguanine and O--propylguanine were separated by this method, 7--propylguanine and 7--propylguanine were not separated. Then we used a h.p.l.c. equipped with a μBonda-pak-C reverse phase column with a linear gradient elution. 7--Propylguanine and O--propylguanine were identified as major reaction products, and 7--opropylguanine and O--propylguanine were identified as minor reaction products. The ratio of O-- and iso-propylguanine/7-- and -propylguanines was 0.73. This ratio is similar to that produced by 1-ethyl-1-nitrosourea and 1--butyl-1-nitrosourea. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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