| Abstrakt: |
The mutagenesis of TA100 and covalent binding of photoactivated aflatoxin B (AFB), aflatoxin B (AFB), aflatoxin G (AFG) and aflatoxin G (AFG) were investigated. Covalent binding levels of 1140, 225, 330 and 8 pmol aflatoxin per μmol nucleotide phosphate were obtained for AFB, AFG, AFB and AFG, respectively, at 50 μM mycotoxin after 2 h of irradiation. Mutant frequencies to histidine prototrophy were 97, 19, 49 and 0 × 10 for AFB, AFG, AFB and AFG respectively, after 2 h irradiation at 100 μM mycotoxin in the surviving fraction of the mutagenized cultures. Toxicity to was 0.59, 0.03, 0.31 and 0 lethal hits under the conditions specified for mutagenesis for AFB, AFG, AFB and AFG, respectively. [ABSTRACT FROM PUBLISHER] |
