| Autor: |
Tweats, D.J., Green, Michael H.L., Muriel, W.J. |
| Zdroj: |
Carcinogenesis; 1981, Vol. 2 Issue 3, p189-194, 6p |
| Abstrakt: |
The gene suppresses the spontaneous inviability of strains of without affecting their repair deficiency. We have taken advantage of this to construct a triple mutant CM871 that combines extreme repair deficiency with near wild-type growth. We have used this strain in conjunction with an isogenic strain WP67 and isogenic wild-type strain WP2 in a differential killing test. Dilute suspensions in buffer of the repair-deficient strains and repair-proficient control are incubated for 2 h at 37°C with test compound in the presence or absence of a S9 metabolising system. Survival is determined by Miles Misra plating. For each strain three 10 μl spots of each of three concentrations of test compound and of the untreated control are placed on a nutrient agar plate. Following overnight incubation survivors are counted. Results with reference compounds are given and the advantages and disadvantages of the test are discussed. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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