| Autor: |
DARSINOS, J. TH., SAMOUILIDOU, E. C., KARLI, J. N., PISTEVOS, A. C., THEODORAKIS, M. G., PAPOUTSIS, C. P., LEVIS, G. M., MOULOPOULOS, S. D. |
| Zdroj: |
European Heart Journal; Oct1995, Vol. 16 Issue 10, p1430-1434, 5p |
| Abstrakt: |
A new method is described for the controlled and specific depletion of calcium from the vascularly perfused heart of experimental animals by means of dialysis, using a pericardial solution. A 30–40ml isotonic phosphate buffer pH7.3 with a low Ca and high Mg concentration (0.2 and 2.7mM respectively) was inserted into the pericardial cavity of anaesthetized dogs and kept therefor 10 or 60 min. The calcium content of the subendocardial and subepicardial halves of the left ventricular wall was similarly decreased to about 70% (P<0.01) within 10 min and to 62% (P<0.001) at 60 min, compared to that of hearts dialysedfor 60 min in a standard solution ofCa 1.2 mM and Mg 1 mM. Calcium content of the myocardium dialysed with low Co and a standard Mg solution decreased to only 75% (P<0.01) at 60 min. Similar changes of calcium were measured in other parts of the heart. An increase in Co concentration in the pericardial solution was observed at the same time as a decrease in calcium in the myocardium. The increase in Ca reached about 0.7 mM at 60 min, but decreased slightly, and finally, fell to 85% of pre-dialysis values at 60 min. It is concluded that this method of myocardial dialysis is effective in reducing myocardial calcium and is influenced by the duration of dialysis and the Mg content of dialysate. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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