| Autor: |
Gore, Angela, Villalobos, Carlos, Frawley, L. |
| Zdroj: |
Endocrine (1355008X); Oct1999, Vol. 11 Issue 2, p131-136, 6p |
| Abstrakt: |
The rate of prolactin (PRL) secretion is influenced by the gender and physiological state of an animal, but little is known about the mechanisms involved. In the present study, we assessed possible contributions of Ca dynamics and PRL gene expression to these differences. This was accomplished by monitoring spontaneous [Ca] changes and PRL promotor-driven reporter activity in pituitary cultures derived from rats comprising a broad spectrum of PRL secretory capacities: male, cycling female, and lactating rats. We found that Ca oscillatory activity exhibited a rank order of lactating > cycling females > males, consistent with the reported secretory capacities of mammotropes from these sources. Interestingly, we observed that the basal level of PRL promotor-driven reporter activity was the same for all three models, but that mammotropes from males were the most responsive to stimulation of PRL gene expression by elevation of [Ca]. Collectively, our findings reveal gender-and state-specific differences in Ca dynamics and induction of PRL gene expression. These likely contribute to reported differences in secretory capacity. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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