| Autor: |
Gatti, Rita, Andreatta, Paolo, Gioia, MariaG., Boschetti, Silvia |
| Předmět: |
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| Zdroj: |
Journal of Liquid Chromatography & Related Technologies; 2010, Vol. 33 Issue 19, p1760-1775, 16p |
| Abstrakt: |
A simple and reliable reversed-phase liquid chromatographic (RP-LC) method was developed and validated to determine simultaneously a glucosamine and chondroitin sulfate equivalent in dietary products. The procedure is based upon the reaction of o-phthaldialdehyde with glucosamine and galactosamine coming from the galactosaminoglycan hydrolysis. The hydrolysis reaction was carried out with hydrochloric acid (7.5 N) at 80°C for 8 hr, whereas, the pre-column derivatization reaction was carried out in alkaline media for 1 min at ambient temperature. The chromatographic separations were performed on a Phenomenex Synergi 4 μ fusion-RP 80 A (250 mm × 3.0 mm i.d.) using a mobile phase consisting of a mixture of sodium acetate buffer (pH 5.9; 0.05 M) and methanol (85:15, v/v). UV-DAD detection at λ = 340 nm was used. Linear responses were observed and the limit of quantitation for both aminosaccharides was about 60 pmol. The intra-day precision (RSD) was ≤1.8% and there was no significant difference between intra- and inter-day data. Recovery studies showed good results (99.3-101.0%) with RSD ranging from 1.1 to 2.1%. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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