| Autor: |
Aaron T. Ohta, Maurice Garcia, Justin K. Valley, Lia Banie, Hsan-Yin Hsu, Arash Jamshidi, Steven L. Neale, Tom Lue, Ming C. Wu |
| Předmět: |
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| Zdroj: |
Lab on a Chip; 12/7/2010, Vol. 10 Issue 23, p3213-3217, 5p |
| Abstrakt: |
Optoelectronic tweezers was used to manipulate human spermatozoa to determine whether their response to OET predicts sperm viability among non-motile sperm. We review the electro-physical basis for how live and dead human spermatozoa respond to OET. The maximal velocity that non-motile spermatozoa could be induced to move by attraction or repulsion to a moving OET field was measured. Viable sperm are attracted to OET fields and can be induced to move at an average maximal velocity of 8.8 ± 4.2 µm s−1, while non-viable sperm are repelled to OET, and are induced to move at an average maximal velocity of −0.8 ± 1.0 µm s−1. Manipulation of the sperm using OET does not appear to result in increased DNA fragmentation, making this a potential method by which to identify viable non-motile sperm for assisted reproductive technologies. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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