QTL Mapping of a High Protein Digestibility Trait in Sorghum bicolor.

Autor: A.Winn, Jennifer, Mason, R. Esten, Robbins, Adriana L., Rooney, William L., Hays, Dirk B.
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Zdroj: International Journal of Plant Genomics; 2009, Vol. 2009, Special section p1-6, 6p, 1 Diagram, 1 Chart, 4 Graphs
Abstrakt: Compared with other cereal grains, Sorghum bicolor shows lower protein digestibility. The low digestibility is thought to result from disulfide cross linking in the β- and γ-kafirins. In contrast, the single recessive high digestibility/high lysine content (HD) mutation which confers greater grain digestibility exists in sorghum that is thought to result from reduced accumulation of γ-kafirin that allows greater access to the high digestible α-kafarin fraction. In an effort to both clearly define the molecular basis for theHD trait and develop tools to improve the introgression of this difficult-to-screen trait, this study focuses on mapping the QTLs linked to this trait. While the HD trait has been defined as a single recessive gene, our results uncovered that two major QTLs on chromosome 1 are associated with protein digestibility-one QTL (locus 1 from the HD parent) unfavorably affects digestibility and one QTL (locus 2 from the HD parent) only 20 cM away favorably affects digestibility. A contrast analysis between genotypic groups at these two loci shows that a higher level of protein digestibility may be obtained when this linkage in repulsion is broken and favorable alleles are allowed to recombine. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index