| Autor: |
Ruwhof, C., van Wamel, A.E.T., van der Valk, L.J.M., Schrier, P.I., van der Laarse, A. |
| Předmět: |
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| Zdroj: |
Archives of Physiology & Biochemistry; Feb2001, Vol. 109 Issue 1, p10, 9p |
| Abstrakt: |
Several studies have demonstrated that static stretchof cardiomyocytes induces cardiomyocyte hypertrophy. We investigated the effectsof cyclic stretch, a more physiological stimulus, on protein synthesis andDNA synthesis of rat ventricular cardiomyocytes and cardiofibroblasts. Further-more,we investigated whether these effects are caused by autocrine mechanisms.In addition, we studied the paracrine influences of stretched cardiofibroblastson cardiomyocyte growth. Short-term cyclic stretch (0–24 h) of cardiomyocytes induced a growthresponse indicative of cardiomyocyte hypertrophy, given the fact that increasedrates of protein synthesis and DNA synthesis were accompanied by an ele-vatedrelease of atrial natriuretic peptide into the culture medium. In cardiofibroblasts,short-term cyclic stretch also induced a growth response as indicated by anincreased rate of protein synthesis and DNA synthesis. Furthermore, incubation of stationary cardiofibroblasts with conditionedmedium derived from stretched cardiofibroblasts revealed an autocrine effectof stretch as illustrated by an increased rate of protein synthesis in stationarycardiofibroblasts. In analogy, there was an autocrine effect of stretch onstationary cardiomyocytes incubated with con-ditioned medium derived fromstretched cardiomyocytes. Moreover, we observed a paracrine effect of theconditioned medium derived from stretched cardiofibroblasts on station-arycardiomyocytes. Thus, short-term cyclic stretch of cardiomyocytes and cardiofibroblastsinduces growth responses that are the result of direct, autocrine, and paracrineeffects. These autocrine/paracrine effects of stretch are most probably dueto release of factors from stretched cells. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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