| Autor: |
Mikhailov, V., Kaminskaya, E., Popov, B., Kuzovatov, S., Skripkina, N., Kosyakova, G., Zaichik, A., Grinchuk, T., Nikolsky, N. |
| Zdroj: |
Cell & Tissue Biology; Oct2010, Vol. 4 Issue 5, p419-423, 5p |
| Abstrakt: |
The purpose of the work was morphological and histochemical examination of tissue differentiation in tumors developed in mdx mice after the intramuscular transplantation of GFP-positive mesenchymal bone-marrow stem cells (MSC-GFP) derived from C576BL/6 transgenic mice and cultivated for 43-45 passages. These cells did not generate tumors in syngeneic adult C57BL/6 mice. The tumors were classified as mesenhymomas, fibrosarcomas, and sarcoma. Adipocyte and chondrocyte clusters, as well as bone areas with erythroid, myeloid, and thrombocyte hematopoiesis and neural tissue with glia cells were observed inside of tumors. Types of tissue tumor differentiation were similar to those described in the literature for MSCs induced to differente in vitro by specific treatment. However, the differentiation spectrum in MSC-GFP-produced tumors was broader than the differentiation of tumors derived from adult mouse MSCs spontaneously transformed or transfected in vitro. The results presented here, along with our previous data, demonstrate that the transfection of stem cells, including totipotent stem cells, with genetic constructs is accompanied by the destabilization of the cell genome, even if the activity of inserted gene (GFP) does not affect general cell functioning. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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