| Autor: |
Löfman, C., Zackrisson, U., Mikuni, M., Block, M., Janson, P. O., Brännström, M. |
| Zdroj: |
Reproductive Sciences; Nov/Dec2002, Vol. 9 Issue 6, p379-385, 7p |
| Abstrakt: |
Objective:We developed an in vivo model to enable observation of dynamic changes in morphology, vascularity, and motility of the rat adnexa.Methods:Immature Sprague-Dawley rats (n = 16) were primed with equine chorionic gonadotrophin (eCG; 15 IU) followed by human chorionic gonadotrophin (hCG; 15 IU) 48 hours later to induce ovulation. The experiments were performed during prolonged (up to 12 hours) thiobarbiturate anesthesia. During laparotony the periovarian bursa was retracted, whereafter the oviductal-ovarian complex was submerged into an organ chamber. Water immersion lenses (4×-40×; final magnification up to 810×) enabled detailed observations that were recorded on Beta-SP videotape.Results:Capillary flow was monitored easily. At the level of the follicle, top blood flow velocity variations (8-10 per minute) were observed in the microvasculature. Ovulations were followed in detail, and oocyte-cumulus complexes were seen later in the oviductal ampulla. Regular contractions in the oviduct were synchronous with the oocyte-cumulus complexes moving back and forth in the oviductal lumen over a distance of about 900 μm. These contractions were more frequent (13-16 per minute) in the postovulatory phase compared with the time before ovulation (9-10 per minute). The oviductal contractions were initiated alternately from either end of the ampulla and were accompanied by a denudation of the oocytes, with a stream of cumulus cells seen moving in an abovarian direction in between conractions.Conclusion:High-magnification video recording in vivo was useful for capturing microcirculatory events as well as structural and functional changes of the ovary and the oviduct. [ABSTRACT FROM PUBLISHER] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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Full text from SpringerLink