| Autor: |
Leila Barani, Ebrahim Vasheghani‑Farahani, Hamideh Aghajani Lazarjani, Sameereh Hashemi‑Najafabadi, Fatemeh Atyabi |
| Předmět: |
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| Zdroj: |
Biotechnology & Applied Biochemistry; Oct2010, Vol. 57 Issue 1, p25-30, 6p |
| Abstrakt: |
The surface modification of Langerhans islets by grafting activated poly(ethylene glycol) on to their capsules in order to prevent immune-system stimulation is a novel method in diabetes cell therapy. In the present study, mPEG [methoxypoly(ethylene glycol)] with two molecular masses of 5 and 10 kDa, activated with SC (succinimidyl carbonate), was grafted on to the surface of pancreatic islets at a polymer concentration of 22 mg/ml. It was found that PEGylated islets were viable and active, and no morphological changes on the collagen capsule of islets were observed. The amount of interleukin-2 secretion from lymphocytes co-cultured with islets PEGylated with mPEG-SC of 5 and 10 kDa was 112.12±23.09 pg/ml and 172.75±27.94 pg/ml respectively. Thus mPEG-SC (SC-activated mPEG) with higher molecular mass was more suitable for camouflaging islets from the immune system. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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